Phase 2 — Core Bioinformatics
Module 07

Sequence Alignment

Map sequencing reads to a reference genome. Understand SAM/BAM formats, alignment flags, and how to extract useful statistics. You cannot do differential expression or variant calling without this step.

Weeks 9–10Timeline
~14 hrsStudy time
FREEAlways
What you'll learn

Topics covered in this module

STAR
HISAT2
SAMtools
SAM/BAM format
featureCounts
IGV

📋 Not sure where this fits? Module 07 is part of the full bioinformatics curriculum — a structured 42-week learning path from Bash to single-cell RNA-seq.

See full curriculum →
Lessons

10 lessons in this module

01Introduction to Sequence AlignmentLive →
02The Reference Genome & IndexingComing soon
03Aligning Reads with STARComing soon
04Aligning Reads with HISAT2Coming soon
05SAM & BAM FormatsComing soon
06Sorting & Indexing BAM Files with samtoolsComing soon
07Alignment QC — flagstat, idxstats, MultiQCComing soon
08Generating Count Matrices with featureCountsComing soon
09Splice-Aware Alignment & Junction DiscoveryComing soon
10Capstone: Full Alignment PipelineComing soon

Lesson 1 is live.

Lesson 1 — Introduction to Sequence Alignment is ready to read now. The remaining 9 lessons are being built, each with exercises, datasets, and a GitHub repository you push as proof of skill. Sign up to be notified as they go live.

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Module 07 of 31