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Phase 4 — Single-cell RNA-seq
Module 28

Trajectory & Pseudotime Analysis

Model cell differentiation and developmental processes — especially relevant in plant biology. Order cells along a developmental path with Monocle3 in R, then model RNA velocity with scVelo in Python.

Week 37Timeline
~7 hrsStudy time
FREEAlways
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What you'll learn

Topics covered in this module

Monocle3
Pseudotime ordering
scVelo
RNA velocity
Spliced / unspliced

📋 Not sure where this fits? Module 28 is part of the full bioinformatics curriculum — a structured 42-week learning path from Bash to single-cell RNA-seq.

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Module 28 of 31